The Epigenetic Factor HP1γ Facilitates Cardiac Differentiation of P19 Embryonal Carcinoma Cell
نویسندگان
چکیده
منابع مشابه
Enhanced Cardiomyogenic Differentiation of P19 Embryonal Carcinoma Stem Cells
BACKGROUND AND OBJECTIVES We investigated the effects of different concentrations of serum, 5-azacytidine, and culture time on the cardiomyogenic differentiation of P19 embryonal carcinoma stem cells in the course of developing an efficient protocol for generating the cardiomyogenic lineage. MATERIALS AND METHODS P19 cells were plated at a density of 1x10(6) cells on 10-cm bacterial dishes fo...
متن کاملRetinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor.
Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferation, survival and differentiation in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, ...
متن کاملpp60src tyrosine kinase modulates P19 embryonal carcinoma cell fate by inhibiting neuronal but not epithelial differentiation
P19 embryonal carcinoma cells provide an in vitro model system to analyze the events involved in neural differentiation. These multipotential stem cells can be induced by retinoic acid (RA) to differentiate into neural cells. We have investigated the ability of several variant forms of the protein-tyrosine kinase (PTK) pp60src to modulate cell fate determination in this system. Normally, P19 ce...
متن کاملDifferentiation of P19 Carcinoma Cell Line into Cardiomyocytes by Oxytocin Hormone
Purpose: The Present study was designed to investigate the OT effects on differentiation of P19 carcinoma cell line into cardiomyocytes. Materials and Methods: P19 carcinoma cell line were cultivated in hanging drops for 2 days to form aggregates termed embryoid bodies (EBs) and in suspension for 5 days. The EBs was treated with oxytocin hormone and DMSO. The EBs were then plated onto gelatin-...
متن کاملInhibition of Rho Kinase Regulates Specification of Early Differentiation Events in P19 Embryonal Carcinoma Stem Cells
BACKGROUND The Rho kinase pathway plays a key role in many early cell/tissue determination events that take place in embryogenesis. Rho and its downstream effector Rho kinase (ROCK) play pivotal roles in cell migration, apoptosis (membrane blebbing), cell proliferation/cell cycle, cell-cell adhesion and gene regulation. We and others have previously demonstrated that inhibition of ROCK blocks e...
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ژورنال
عنوان ژورنال: Journal of Arrhythmia
سال: 2011
ISSN: 1880-4276,1883-2148
DOI: 10.4020/jhrs.27.op04_5